Carbamoyl phosphate synthetase (glutamine-hydrolysing) (EC 6.3.5.5) is an enzyme that catalyzes the reactions that produce carbamoyl phosphate in the cytosol (as opposed to type I, which functions in the mitochondria).
Its systemic name is hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating).
[1][2][3][4][5][6][7][8] In pyrimidine biosynthesis, it serves as the rate-limiting enzyme and catalyzes the following reaction: It is activated by ATP and PRPP[9] and it is inhibited by UTP (Uridine triphosphate)[10] Neither CPSI nor CPSII require biotin as a coenzyme, as seen with most carboxylation reactions.
It is one of the four functional enzymatic domains coded by the CAD gene.
This EC 6.3 enzyme-related article is a stub.