Following the tragic death of Selena Donovan at the end of Series 21, the staff of Holby City's Emergency Department find themselves thrown back into the thick of the action.
Series 22 saw the arrival of many new characters to the team which included F2s Ruth Winters (Georgia Taylor) and Toby De Silva (Matthew Needham), Consultants Adam Trueman (Tristan Gemmill) and Zoe Hanna (Sunetra Sarker), Porter Big Mac (Charles Dale), Receptionist Noel Garcia (Tony Marshall), Paramedics Snezana Lalovic (Ivana Basic) and Curtis Cooper (Abdul Salis), Orthopaedic Consultant Sean Anderson (Richard Dillane) and his wife, Staff Nurse Jessica Harrison (Gillian Kearney) and Clinical Nurse Manager Simon Tanner (Paul Fox).
Dramatic changes in Series 22 included the departures of many beloved characters such as the long serving Josh Griffiths (Ian Bleasdale) and Harry Harper (Simon MacCorkindale) and as the series drew to a close it seemed yet another loved character was due to leave as Dr. Maggie Coldwell (Susan Cookson) quit Holby following a vicious court case.
Toby makes several initial mistakes with one being letting a patient that has overdosed on paracetamol and another being that he fails to insert a simple IV line after 6 years of training.
Note: First appearance of Ruth Winters and Toby De Silva as F2 Doctors Selena and Nathan's baby is recovering in the ED, Angel, has been off the ventilator for the past day.
Meanwhile the daughter of one of Guppy's patients who died is desperate to speak to him but he gives her the cold shoulder and demands that Alice deals with it, much to Charlie's disgust.
Harry returns as the new Consultant manager much to everyone's, especially Nathan's, shock.The train and station scenes were filmed on location at Wirksworth on the Ecclesbourne Valley Railway.
Meanwhile a family are walking through the woods when a car careers off the road through a field, runs down the mother and crushes the granddad into a tractor all the while with the young daughter staring on.
As the nurses and Big Mac pop open a bottle of champagne at midnight, Tess storms in sending them all back to work.
After Abs suffers a gun shot wound after trying to calm down Dean, Tess begins to prepare the department for the impending rush of patients from the shooting.
Tess warns Ruth and Nadia that one of them is lying and that she wants the truth by the end of the day or there will be a formal investigation of how the patient was injured in the hospital.
It turns out that the woman wanted the train to amputate her leg so Zoe diagnosed her with BIID (Body Integrity Identity Disorder, desire for elective impairment).
Maggie's daughter begins her contractions, Jeff is injured in a pub brawl making Greg consider whether he should resign after Dixie calls him a liability.
After the incident the team go to the pub to drown their sorrows where Maggie feels bad after Ruth previously asked her for guidance to which she refused to help.
Dixie and Jeff do not notice the girl in the woods and leave to assist Toby, presuming the call is a hoax, only to find the stabbed man in the middle of the road.
A young teenage boy, Daz, hangs around outside a house until chased away by the owner and later has a panic attack on a bus and jumps out of the emergency exit window in front of a car.
He seems to have traumatic amnesia and, when he turns out to be allergic to latex, Sean remembers him as the only survivor of a multi-car pile-up in which his parents and sister were killed, which he has repressed; he now lives with his uncle Terry.
Husband and father Khalid has lost his job and is secretly working as a taxi driver but Kay thinks he is having an affair and tries to burn his things.
Parents Mike and Gill pick up their daughter Rose, who has Down's syndrome, from a care centre and learn she is dating one of the other attendees, David, who gives her a letter.
Lucy, a woman who came in with stomach pains, is discovered to be pregnant but insists she hasn't had sex since her husband left her over a year ago.
Her lodger Justin, who has a scarred face from saving a colleague's life in Afghanistan, admits they had sex while she was drunk and she doesn't remember.
Ruths and Abs realise he has foreign accent syndrome and Sally hit him to try and change him back, managing to reconcile the couple.
Tom, an author at the convention, is pleased that George, a fan who won a competition to meet him, is an attractive blonde woman and invites her for dinner.
Maggie takes Dixie and Ian to check out her house and finds an old man and dog dead and an elderly woman in a bad way because of a leak.
A father, Rizwan, is trying to leave for the airport with his teenage daughter Nazrin before his wife Trish gets home and pulls out in front of Stacey, causing a crash.
Sean and Jessica are having liaisons with Zoe and Adam respectively and miss a call from the childminder saying she can't pick up Lucas from school.
She finds an air gun in her son Goran's bag and, during a struggle, Branka is shot in the face and Josie, a patient who has been in a coma for four years, is knocked out of bed.
Jeff follows Snezana to a farm and finds her treating immigrants, including giving drugs and injections she isn't qualified for.
Luke's mother arranges for a journalist to shadow him as a promising young doctor but he points the man towards Ruth when he sees Holly in reception.
Marko and several other immigrants are working at a quarry where the boss, Greyling, molests Mina, the wife of worker Milosch, in his office.