Trequanda is a comune (municipality) in the Province of Siena in the Italian region Tuscany, located about 70 kilometres (43 mi) southeast of Florence and about 30 kilometres (19 mi) southeast of Siena.

Trequanda borders the following municipalities: Asciano, Pienza, Rapolano Terme, San Giovanni d'Asso, Sinalunga and Torrita di Siena and consists of the following villages: Trequanda, Castelmuzio and Petroio.

It houses an Ascension attributed to Il Sodoma and a terracotta of "Madonna with Child" attributed to Andrea Sansovino.

The high altar (15th century) is by Giovanni di Paolo.

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