[1] Further, vaccination against the alpha toxin toxoid protects mice against C. perfringens gas gangrene.

[2] As a result, knowledge about the function of this particular protein greatly aids understanding of myonecrosis.

There is significant homology with phospholipase C enzymes from Bacillus cereus, C. bifermentans, and Listeria monocytogenes.

[3] The C terminal domain shows similarity with non-bacterial enzymes such as pancreatic lipase, soybean lipoxygenase, and synaptotagmin I.

This property allows hydrolysis of phospholipids such as phosphatidyl choline, mimicking endogenous phospholipase C. The hydrolysis of phosphatidyl choline produces diacylglycerol, which activates a variety of second messenger pathways.