Colorimetry (chemical method)

In physical and analytical chemistry, colorimetry or colourimetry is a technique used to determine the concentration of colored compounds in solution.

There are also electronic automated colorimeters; before these machines are used, they must be calibrated with a cuvette containing the control solution.

A colorimeter is a device used to test the concentration of a solution by measuring its absorbance of a specific wavelength of light.

Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte.

They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes.