The following is the qualification system and qualified countries for the cycling at the 2023 Pan American Games competition in Santiago, Chile.
Chile as host nation, was automatically awarded the maximum quota of 34 spots.
Qualification will be done using the 2021 Junior Pan American Games and the UCI rankings as of December 31, 2022.
[1] A maximum of 18 male and 18 female athletes will be allowed to compete in mountain biking.
[1] A maximum of 94 male and 93 female athletes will be allowed to compete in the road and track cycling events.