The following is the qualification system for the triathlon at the 2023 Pan American Games event and qualified athletes/quotas.
A nation may enter a maximum of 6 triathletes (three per gender),with the exception of the winners of the 2021 Junior Pan American Games.
The host nation (Chile) automatically qualified four athletes (two per gender).
[1] The top placed mixed relay team not yet qualified at the 2022 South American Games and 2023 Central American and Caribbean Games will each qualify two athletes per gender.
A country may enter the mixed relay competition if it has qualified at least two male and two female triathletes.