Information based on LPSN 2021 Cytobacillus is a genus of rod-shaped bacteria that stain either Gram-positive or Gram-variable in the family Bacillaceae within the order Bacillales.

[6] Many studies have used phylogenetic and comparative genomic analyses as a means towards clarifying the complicated taxonomic relationships within Bacillus, resulting in the transfer of many species into novel genera such as Alkalihalobacillus,[1] Virigibacillus,[7] Brevibacillus,[8] Solibacillus[9] and Evansella.

[1] These CSIs were identified through the analysis of genome sequences from Cytobacillus species and provide novel molecular means to differentiate this genus from other Bacillaceae genera.

[10] Members of Cytobacillus are observed to group together in a monophyletic clade in phylogenetic trees created based on different datasets of conserved proteins as well as 16S rRNA genome sequences.

It is important to note as genome and strain information becomes available, it is necessary to conduct additional analyses of these classifications to account for any required amendments.