DNA polymerase III holoenzyme

Being the primary holoenzyme involved in replication activity, the DNA Pol III holoenzyme also has proofreading capabilities that corrects replication mistakes by means of exonuclease activity reading 3'→5' and synthesizing 5'→3'.

The replisome is composed of the following: DNA polymerase III synthesizes base pairs at a rate of around 1000 nucleotides per second.

[3] DNA Pol III activity begins after strand separation at the origin of replication.

[citation needed] After replication of the desired region, the RNA primer is removed by DNA polymerase I via the process of nick translation.

The removal of the RNA primer allows DNA ligase to ligate the DNA-DNA nick between the new fragment and the previous strand.

Schematic picture of DNA polymerase III* (with subunits). This is the old textbook "trombone model" with two units of Pol III.