The institute consists of three service divisions: the academic division - consisting of the colleges and a senior high school (all of which are only medicine and allied health-related courses), its teaching hospital - De La Salle University Medical Center, and a research facility - Angelo King Medical Research Center.

Originally named Emilio Aguinaldo College of Medicine, the school opened in 1979 under the management of the University Physician’s Foundation, Inc. with the Jose P. Rizal National Medical Research Center as its initial teaching hospital.

By June 1995, the complex consisting of the College of Medicine, University Medical Center, College of Physical Therapy, and Research Services was named De La Salle University – Health Sciences Campus or DLSU-HSC under the ownership and administration of the Saint Br.

[1] In October 2019, DLSMHSI became an official member of the ASEAN University Network-Quality Assurance (AUN-QA) as confirmed by Banjong Ujjin, the Senior Programme Officer of the AUN Secretariat Team.

[5] On December 4, 2023, DLSMHSI proudly introduced its latest symbol of pride, ‘The Green Sentinels’, which represents the institution's commitment to excellence and service in the field of medical and health sciences.

Christian Alviar and Lander Angelo Pua were celebrated for their winning entries in the Moniker and Mascot-making competition.

[6] DLSMHSI offers academic programs through its nine colleges and a special health sciences senior high school.

[7][8][9][10] DLSMHSI accepts walk-in, online, and foreign applicants for the senior high school, undergraduate, and medicine programs.

Medicine program applicants, however, should have taken the National Medical Admission Test (NMAT) and have a ranking score of 90th percentile or higher.

[14] The De La Salle University Medical Center ('DLSUMC; also known as UMC) acts as the teaching hospital of DLSMHSI.

Modern equipment continued to be acquired to make DLSUMC comparable with any of the large hospitals in Metro Manila.

August 28, 1997, marked the inauguration of the 10-story hospital annex building, the De La Salle University-Doña Teodorica Favis Vda.