Dihydroorotase (EC 3.5.2.3, carbamoylaspartic dehydrase, dihydroorotate hydrolase) is an enzyme which converts carbamoyl aspartic acid into 4,5-dihydroorotic acid in the biosynthesis of pyrimidines.

[1][2] It forms a multifunctional enzyme with carbamoyl phosphate synthetase and aspartate transcarbamoylase.

Dihydroorotase is a zinc metalloenzyme.

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