Discontinuous electrophoresis

[2][1] This method produces high resolution and good band definition.

It is widely used technique for separating proteins according to size and charge.

Proteins move towards anode slowly at constant speed till they reach limit of separation gel.

Suddenly, frictional resistance increases but glycine is not affected and it passes the proteins and becomes highly charged in resolving zone.

Proteins present in homogeneous buffer start to separate based on principles of zone electrophoresis.