Electroblotting
Electroblotting is a method in molecular biology/biochemistry/immunogenetics to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose, after gel electrophoresis.
[1][4] This technique relies upon current and a transfer buffer solution to drive proteins or nucleic acids onto a membrane.
A stack is put together in the following order from cathode to anode: sponge | three sheets of filter paper soaked in transfer buffer | gel | PVDF or nitrocellulose membrane | three sheets of filter paper soaked in transfer buffer | sponge.
It is a necessity that the membrane is located between the gel and the positively charged anode, as the current and sample will be moving in that direction.
Because the proteins may retain or regain part of their structure during blotting they may react with specific antibodies giving rise to the term immunoblotting.
