In enzymology, a deoxycytidylate 5-hydroxymethyltransferase (EC 2.1.2.8) is an enzyme that catalyzes the chemical reaction The 3 substrates of this enzyme are 5,10-methylenetetrahydrofolate, H2O, and deoxycytidylate, whereas its two products are tetrahydrofolate and 5-hydroxymethyldeoxycytidylate.
This enzyme belongs to the family of transferases that transfer one-carbon groups, specifically the hydroxymethyl-, formyl- and related transferases.
The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:deoxycytidylate 5-hydroxymethyltransferase.
This enzyme participates in pyrimidine metabolism and one carbon pool by folate.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1B49, 1B5D, and 1B5E.