In enzymology, an undecaprenyl-diphosphatase (EC 3.6.1.27) is an enzyme that catalyzes the chemical reaction Thus, the two substrates of this enzyme are undecaprenyl diphosphate and H2O, whereas its two products are undecaprenyl phosphate and phosphate.
In many bacteria, this enzyme is a membrane protein that participates in peptidoglycan biosynthesis.
[2] Note: The enzyme Uppp/BacA (EC 3.6.1.27) has occasionally been incorrectly termed an "undecaprenol kinase".
[3] However, that name should be reserved for a distinct enzyme (EC 2.7.1.66), which catalyses the addition of a phosphate group from ATP to undecaprenol (C55-isoprenyl alcohol).
X-ray crystal structures of the membrane-form of the enzyme from E. coli[4][5] are available (PDB IDs: 5OON,[6] 6CB2[7]).