He studied medicine in Cherry Valley, New York with Dr. Joseph White, a well-known physician of the time.

Dr. Ebenezer Johnson was elected the first mayor of Buffalo with a salary of $250 per year and his political affiliation was Democrat-Republican.

It was surrounded by a high fence and he allowed wild animals to live freely within its limits.

The property included fruit orchards, vegetable gardens, flower beds, and elm trees.

[2] After his second term, he moved to Tellico Plains, Tennessee, where he owned an iron ore mine with his brother, Elisha Johnson, who was a former mayor of Rochester, New York.