Electroelution

Electroelution is a method used to extract a nucleic acid or a protein sample from an electrophoresis gel by applying a negative current in the plane of the smallest dimension of the gel, drawing the macromolecule to the surface for extraction and subsequent analysis.

[3][4] Using this method, DNA fragments can be recovered from a particular region of agarose or polyacrylamide gels.

The DNA fragments are recovered from this buffer and purified, using phenol–chloroform extraction followed by ethanol precipitation.

This method is simple, rapid and yields high recovery (75%) of DNA fragments from gel pieces.

[3] Proteins are continuously eluted from a gel column and separated in different fractions according to their isoelectric point.

Equipment for bioanalytical continuous- elution gel electrophoresis : electrophoresis chamber, peristaltic pump , fraction collector, buffer recirculation pump and UV detector (in a refrigerator ), power supply and recorder (on a table). [ 1 ]