Eugene is an unincorporated area in Dixie County, Florida.

[2] A 1967 state geology report noted Pamlico Dune near Eugene on U.S. Highway 27.

[3] Eugene is south of Cross City and North of Old Town, Florida on U.S. Route 98 in Florida.

The Nature Coast State Trail also passes by.

This article about a location in Dixie County, Florida is a stub.