It runs from northwestern Tampa to Plant City, and parallels County Road 574 west of Mango.

The next two major crossings are a diamond interchange with Interstate 275 (I-275) and then State Road 45 (Nebraska Avenue).

East of here, the road consists of residences, local businesses and churches, and most notably the headquarters of radio station WMNF 88.5 FM.

East of here, the Florida State Fairgrounds and a gas station can be found on the northwest corner of US 301 in Mango.

In Plant City, SR 574 becomes Sammonds Road, then SR 574 moves onto West Reynolds Street at a sharp reverse curve However neither of these names appear on street-name signs until the route moves off of Sammonds Road.

When SR 574 returns eastward it runs along the south side of the ground of the Florida Strawberry Festival.