It begins at the MacDill Air Force Base entrance in South Tampa and ends by merging with US 41 just north of the Pasco County border.

Dale Mabry Highway has many items of interest, including Raymond James Stadium, George M. Steinbrenner Field, Tampa International Airport, the Dale Mabry branch of the Hillsborough Community College, Plant High School, Gaither High School, shopping centers, car dealerships and restaurants.

The southern portion of Dale Mabry Highway between MacDill AFB and Gandy Boulevard (US 92, SR 600) is designated as State Road 573 for its entire length.

In 2002, a roundabout was constructed just north of the main gate to MacDill as part of a security enhancement and road improvement project for the base.

The section of Dale Mabry Highway between Gandy Boulevard and Hillsborough Avenue is signed as U.S. Route 92 (State Road 600).

This stretch of Dale Mabry Highway snakes through several south Tampa business areas and a residential district before approaching Raymond James Stadium with the SR 685 concurrency ending at Henderson Boulevard.

Additionally a 1950s era overpass stood over a CSX rail corridor just north of the Lee Roy Selmon Crosstown Expressway.

The overpass was torn down in the late 1990s and replaced with an at-grade railroad crossing as part of a widening project which increased that section to four lanes.

[3] Dale Mabry runs along what was originally called Vera Ave. according to land plots from the Hillsborough County Property Appraiser.