Fluorescence polarization immunoassay

Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample.

FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the requirement of separation or washing steps.

[2] From 1925 to 1926 Francis Perrin detailed a quantitative theory for fluorescence polarization in multiple significant publications which remain relevant to this day.

[4] FPIA quantifies the change in fluorescence polarization of reaction mixtures of fluorescent-labelled tracer, sample antigen, and defined antibody.

[7] A conventional FPIA follows the procedure below: FPIA has emerged as a viable technique for quantification of small molecules in mixtures, including: pesticides,[8] mycotoxins[9] in food, pharmaceutical compounds in wastewater,[10] metabolites in urine and serum indicative of drug use (cannabinoids, amphetamines, barbiturates, cocaine, benzodiazepines, methadone, opiates, and PCP),[11][12] and various small molecule toxins.