In enzymology, a formamidase (EC 3.5.1.49) is an enzyme that catalyzes the chemical reaction Thus, the two substrates of this enzyme are formamide and H2O, whereas its two products are formate and NH3.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides.

The systematic name of this enzyme class is formamide amidohydrolase.

As of late 2007, four structures have been solved for this class of enzymes, with PDB accession codes 2DYU, 2DYV, 2E2K, and 2E2L.

This EC 3.5 enzyme-related article is a stub.