SR 169 begins at an intersection with US 341/SR 27 in Jesup, in Wayne County.

It curves to the northwest and then heads northward, until it enters Evans County.

The highway continues to the north, intersects SR 169 Spur, and curves to the northeast.

It begins at an intersection with the SR 169 mainline north of Bellville.

It heads north-northeast and curves to meet its northern terminus, at an intersection with SR 129 about 0.5 miles (0.80 km) later.