It runs south-to-north through portions of Lee and Sumter counties.

It heads northeast from Leesburg to an intersection with SR 377 (Lee Street).

Shortly after entering the county, it reaches Leslie.

The three highways run concurrent east to nearby De Soto.

It runs north through rural parts of the county, broken up by SR 27 (Vienna Road), until it meets it northern terminus, an intersection with SR 49 southeast of Andersonville.