SR 313 begins at an intersection with US 82/SR 520 in Sylvester.

The route heads north-northwest through rural parts of the county, and along the way, passes through the unincorporated community of Isabella.

After Isballa, SR 313 continues heading north-northwest until it meets SR 32 in the unincorporated community of Doles.

The highway continues north-northwest, and curves to the northwest just before meeting its northern terminus, an intersection with SR 300 in Warwick.

The entire route is in Worth County.