The highway connects the Florida state line southeast of Lake Park to the Griffin area, via Valdosta, Tifton, Cordele, Perry, and Barnesville.

US 41/SR 7/SR 31 travels to the north-northeast on Inner Perimeter Road on what is essentially a bypass of the main part of Valdosta.

The four highways travel to the north-northwest and curve to the northwest just before reaching East Park Avenue.

On the southwestern edge of Henry Tift Myers Airport, they begin a concurrency with SR 125.

After traveling through Chula, and passing southwest of the Golf Club of South Georgia, they enter Turner County.

Approximately 0.5 miles (0.80 km) northwest of the county line, the two highways cross under, but don't have an interchange with, I-75.

After traveling on the west side of the Flat Creek Public Fishing Area, they enter Perry.

They curve to the southeast on Golden Isles Parkway, before US 41/SR 7/SR 127 departs to the northeast on General Courtney Hodges Boulevard.

In the main part of the city, they turn to the northeast and intersect the western terminus of SR 7 Conn. (East Church Street).

Approximately 500 feet (150 m) later, they begin a curve to the west and intersect SR 49 (North Camellia Boulevard).

The concurrent routes curve to the north-northwest, then to the west, and to the northwest before intersecting SR 49 Conn./SR 540 (Fall Line Freeway).

After traveling through rural portions of the county, the concurrency curves to a nearly due-north routing and enters Roberta.

[1] US 341/SR 7 travels along the western edge of Deer Trail Country Club and continue to the north-northwest.

[1] The following portions of SR 7 are part of the National Highway System, a system of roadways important to the nation's economy, defense, and mobility: SR 7 was established at least as early as 1919 on what is essentially its current path from the Florida state line southwest of Statenville to Griffin.

Two segments were under construction: a portion in the south-southeast part of Tifton and from the approximate location of Arabi to Cordele.

[10][11] By the end of 1929, two segments had a completed hard surface: from Florida to Perry and a portion in the southeast part of Fort Valley.

A very small portion from just south of the Crawford–Monroe county line had a sand clay or top soil surface.

[14][15] In February 1932, the entire Crawford County portion had a sand clay or top soil surface.

[30][31] In 1967, US 341/SR 7 in the Barnesville area was shifted southwest onto the southern part of this planned extension's path, along with SR 333.

is a 7.0-mile-long (11.3 km) business route of SR 7 that exists entirely within the west-central part of Lowndes County.

The business routes travel to the northwest on South Patterson Street and pass just to the southwest of Payton Park.

Just north of Olympic Park, the southbound and northbound lanes split onto one-way pairs.

Just past East Park Avenue, they curve to the north-northwest and intersect the southern terminus of SR 125 (Bemiss Road).

Slightly more than 400 feet (120 m) later, the two highways pass by South Georgia Medical Center and then Billy Grant Baseball Field.

is a 2.8-mile-long (4.5 km) alternate route of SR 7 that exists entirely within the west-central part of Lowndes County and travels completely within the city limits of Valdosta.

Within the university, the highway curves to a more due-north orientation and passes just to the west of Drexel Park.

Next to the Five Points Shopping Center, it meets its northern terminus, a second intersection with the southbound lanes of US 41 Bus./SR 7 Bus.

SR 7 Spur begins at a point of General Courtney Hodges Boulevard near where US 41/SR 7 formerly crossed over Interstate 75 (I-75) in the southern part of Perry.

SR 7 Conn. begins at an intersection with US 341/SR 7 (Oakland Heights Parkway) in the central part of Fort Valley.

[30][31] In 1967, US 341/SR 7 in the Barnesville area was shifted southwest onto the southern part of this planned extension's path, along with SR 333.