GFP-cDNA

The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy.

The cDNAs of novel identified Open Reading Frames(ORF) are tagged with Green Fluorescent Protein (GFP) and expressed in eukaryotic cells.

In this respect those that utilise recombination cloning (Gateway of Invitrogen or Creator of BD Biosciences) have proved to be the most suitable.

This maximises the possibility of correctly ascertaining the localisation, since the presence of GFP may mask targeting signals that may be present at one end of the native protein.

For those ORFs where the two fusions do not give a similar localisation pattern, a series of other criteria, including bioinformatic predictions, are considered.

In addition, use the search window on the entry site to find proteins containing features or motifs of particular interest to you that have been localised in this project.