Gibson assembly

Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction.

It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.

The technology is more efficient than manual plasmid genetic recombination methods, but remains expensive as it is still under patent.

[3] The method can simultaneously combine up to 15 DNA fragments based on sequence identity.

In this method, fragments and a master mix of enzymes are combined and the entire mixture is incubated at 50 °C for up to one hour.