She married Robert III de La Marck in 1510, with whom she had a son, Robert IV de La Marck (1512–1556), Duke of Bouillon, Prince of Sedan and Marshal of France.
Guillemette de Sarrebruck had a long career at the royal court of France.
She served as dame or dame d'honneur (lady-in-waiting) to three queens of France, Anne de Bretagne, Eleanor of Austria, and Catherine de Médici.
In 1559, she was appointed head lady-in-waiting or Première dame d'honneur to queen Mary Stuart of France.
Her tenure ended after Mary Stuart was widowed in 1560, and returned to Scotland the following year.