Generally, the alpha subunit binds membrane-bound effector proteins for the downstream signaling cascade, but the beta-gamma complex can carry out this function also.
[2] Reconstitution experiments carried out in the early 1980s showed that purified Gα subunits can directly activate effector enzymes.
For example, in adipose tissues, two different G-proteins with interchangeable beta-gamma complexes are used to activate or inhibit adenylyl cyclase.
This nomenclature is based on their sequence homologies:[6] The β and γ subunits are closely bound to one another and are referred to as the G beta-gamma complex.
The free Gβγ complex can act as a signaling molecule itself, by activating other second messengers or by gating ion channels directly.
Gβγ complexes bound to muscarinic acetylcholine receptors, on the other hand, directly open G protein-coupled inward rectifying potassium channels (GIRKs).