I-TASSER (Iterative Threading ASSEmbly Refinement) is a bioinformatics method for predicting three-dimensional structure model of protein molecules from amino acid sequences.

[1] It detects structure templates from the Protein Data Bank by a technique called fold recognition (or threading).

I-TASSER is one of the most successful protein structure prediction methods in the community-wide CASP experiments.

[2][3] It has an on-line server built in the Yang Zhang Lab at the University of Michigan, Ann Arbor, allowing users to submit sequences and obtain structure and function predictions.

[1] The pipeline consists of six consecutive steps: The I-TASSER server allows users to generate automatically protein structure and function predictions.