The resulting INCYPRO-crosslinked proteins are more stable at elevated temperature and in presence of chemical denaturants.
[6] For example, INCYPRO can involve the introduction of three spatially aligned and solvent-accessible cysteines into the protein that are then reacted with a tris-electrophilic agent.
[6] An early example, involved the stabilization of the transpeptidase Sortase A which resulted in INCYPRO-stabilized variants with activity under elevated temperature and in the presence of guanidinium chloride.
[1][5] INCYPRO has also been applied to stabilize the human adaptor KIX domain utilizing different crosslinker molecules.
[2] In addition, a number of homo-trimeric protein complexes was stabilized including the Pseudomonas fluorescens esterase (PFE) and an Enoyl-CoA hydratase.