The Isesaki Line can refer to the entire section between Asakusa - Isesaki and Oshiage - Hikifune, but from March 2012, the 41.0 km (25.5 mi) section south of Tōbu-Dōbutsu-Kōen was branded as the Tobu Skytree Line in conjunction with the opening of the Tokyo Skytree tower.

The first section of the Isesaki Line was opened by the present company in 1899 between Kita-Senju and Kuki utilising steam motive power.

The sole connection was with the Jōban Line at Kitasenju, which offered poor access to central Tokyo.

To solve the inefficiencies of transfers at Kitasenju and notoriously narrow Asakusa, in 1962, the Hibiya Line of the then Teito Rapid Transport Authority (帝都高速度交通営団, Teito Kōsokudo Kōtsū Eidan), known as TRTA, present Tokyo Metro) was built, connecting at Kitasenju.

In 2003, the company built new tracks from Hikifune to connect at Oshiage, officially an annex station of Tokyo Skytree.