[2][3] Another set of three archers qualified for the women's events by virtue of the nation's podium finish in the team recurve competition at the 2016 Archery World Cup meet in Antalya, Turkey.

A day later, high jumper and London 2012 Olympian Gianmarco Tamberi was forced to withdraw from the team after sustaining a partial rupture of his ankle ligament shortly upon clearing a national record of 2.39 m in Monaco.

Having previously competed for Switzerland in Beijing 2008, Jeanine Cicognini claimed her second Olympic spot as one of top 34 individual shuttlers in the BWF World Rankings as of 5 May 2016.

[11][12] Three further boxers, including London 2012 Olympians Manuel Cappai and bronze medalist Vincenzo Mangiacapre, claimed their Olympic spots at the 2016 European Qualification Tournament in Samsun, Turkey.

[13] Meanwhile, Guido Vianello secured an additional place on the Italian roster as the winner and sole recipient of the men's super heavyweight division at the 2016 AIBA World Qualifying Tournament in Baku, Azerbaijan.

[14] Carmine Tommasone completed the nation's boxing lineup with his box-off victory at the 2016 APB and WSB Olympic Qualifier in Vargas, Venezuela.

[17] Giovanni de Gennaro and Stefanie Horn were selected to the Italian team on May 15, 2016, as a result of their performances at the 2016 European Canoe Slalom Championships.

[18] Italian canoeists have qualified two boats in each of the following distances for the Games by virtue of a top two finish at the 2016 European Qualification Regatta in Duisburg, Germany.

[19] As a response to the "multiple positive" cases on doping that provoked a one-year suspension for Belarus and Romania, the men's K-4 1000 m crew (Crenna, Dressino, Ricchetti, & Ripamonti) received a spare berth from the International Canoe Federation for the Italians.

[23] Following the completion of the 2016 UCI Track Cycling World Championships, Italian riders have accumulated spots in the women's team pursuit, as well as the men's omnium.

The mountain biking team, highlighted by London 2012 bronze medalist Marco Aurelio Fontana, was named to the Olympic roster on June 1, 2016.

[29][30] Multiple Olympic men's sabre medalists Diego Occhiuzzi and Aldo Montano, along with London 2012 champion Elisa Di Francisca and runner-up Arianna Errigo, both in women's foil, claimed their spots on the Italian team by finishing among the top 14 fencers in the FIE Adjusted Official Rankings, while Rossella Fiamingo did the same feat in women's épée as the highest-ranked fencer coming from the Europe zone.

Elios Manzi, Fabio Basile, Odette Giuffrida, and London 2012 Olympian Edwige Gwend were ranked among the top 22 eligible judokas for men and top 14 for women in the IJF World Ranking List of May 30, 2016, while Matteo Marconcini at men's half-middleweight (81 kg) earned a continental quota spot from the European region, as the highest-ranked Italian judoka outside of direct qualifying position.

[42][43] On June 30, 2016, the International Judo Federation awarded a spare Olympic berth freed up by Palau to Valentina Moscatt at women's extra-lightweight (48 kg), as one of the highest-ranked eligible judokas, not yet qualified, across all genders and continents on the list.

[49] Following their podium finish at the 2015 49er & 49erFX World Championships, 2012 Olympians Giulia Conti and Francesca Clapcich became the first Italian sailors to be selected for Rio regatta.

[50] By the end of 2015, two more crews joined Conti and Clapcich in the Italian Olympic sailing squad: Vittorio Bissaro and Silvia Sicouri (Nacra 17), and Flavia Tartaglini (RS:X).

[51] Two further sailors (Camboni & Marrai) sealed their Olympic spots at the Princess Sofia Trophy regatta, while the remaining sailing crews (except the men's 470) rounded out the Italian selection on the last week of May 2016.

[61] Italy has fielded a squad of nine synchronized swimmers to compete in both the women's team and duet routine by virtue of their third-place finish at the FINA Olympic test event in Rio de Janeiro.

[64][65] On June 30, 2016, the International Tennis Federation awarded one of the Olympic women's singles places to three-time Olympian Francesca Schiavone, as Italy's top-ranked player outside of direct qualifying position, but she decided to reject the invitation.

London 2012 Olympians Alessandro Fabian, Davide Uccellari, and Annamaria Mazzetti, along with Charlotte Bonin, were ranked among the top 43 eligible triathletes each in the men's and women's event, respectively, based on the ITU Olympic Qualification List as of May 15, 2016.

The Italian Olympic Committee selected London 2012 Olympian Mirco Scarantino and rookie Giorgia Bordignon to the weightlifting team for the Games.