K-65 is an approximately 11.1-mile-long (17.9 km) west–east state highway located entirely within Bourbon County in eastern Kansas.

The highway continues roughly 0.9 miles (1.4 km) then passes under a transmission line and then intersects 95th Street by Northway Cemetery.

K-65 continues through mostly open pastures for roughly 1.9 miles (3.1 km) then curves north as it briefly parallels Little Osage River.

The roadway continues north for 0.75 miles (1.21 km) then curves east as it crosses Opossum Creek, a tributary of the Little Osage River.

Then in an October 9, 1957 resolution K-31 was rerouted further east on K-52 from Blue Mound, bypassing Osage, then left K-52 and went directly south to Mapleton.

[16] The section of K-65 east of Xenia by the Little Osage River, has had to close briefly numerous times due to flooding.

[18] On November 4, 1974, the section of K-65 by the river was closed due to flooding, when over seven inches (18 cm) of rain fell in parts of southeast Kansas.

[19] On October 3, 1986, heavy rain from remnants of Hurricane Paine caused flooding that closed a section of the roadway at the river crossing.