In 1862 he took his doctoral examination in medicine, and in the same year acquired his postdoctoral qualification in biochemistry and was in charge of what was then called the “zoochemisches laboratorium”.
[2] While still in Leipzig, Huppert became professor ordinarius in 1872, but the same year he accepted a call to Prague for the newly established chair of applied medicinal chemistry at Charles University, and became to first to teach the new discipline in 1872.
Among the many issues in physiological and pathological chemistry he worked on, he was mainly concerned with the formation of the body's own substances like hemoglobin, bilirubin and glycogen.
Among his pupils were eminent researchers, e. g. Rudolf von Jaksch, Otto Kahler and Franz Hofmeister.
Huppert was engaged in a committee of the faculty concerned with restructuring the program of medical study.