[3] Both inhibitors have significant anti-nutritive effects in the body, affecting digestion by hindering protein hydrolysis and activation of other enzymes in the gut.

The active inhibitory site containing the scissile bond is located in the loop between beta-strands 4 and 5 in STI and ETI.

They bind strongly to trypsin, blocking its active site and instantly forming a highly stable adduct and halting digestion of certain proteins.

In both instances, after a week the rats showed a dose-related increase in pancreas weight due to both hyperplasia and hypertrophy.

[7] This indicates that long-term consumption of a diet high in soy with strong trypsin inhibitor activity may produce unwanted effects in humans as well.

Loops of STI were randomized and selected by phage display for binding to a target of interest (a toxin from Clostridioides difficile).

Further research is still necessary to determine things such as the method of delivery for this natural anti-carcinogen, as well as performing extensive clinical trials in this area.