It is named Bull Run in a section of downtown Lewisburg, including as it passes beside Hufnagle Park.

[1] The concentration of dissolved oxygen in the streams of the Limestone Run watershed ranges from 5.31 to 12.7 milligrams per liter.

[1] The concentration of total dissolved solids in the watershed of Limestone Run is approximately 0.289 to 0.29 milligrams per liter near its headwaters.

In the middle reaches of the stream, the total dissolved solid concentration is approximately 0.38 to 0.388 milligrams per liter.

The total dissolved solids concentration on Limestone Run's tributaries ranges from 0.262 to 0.378 milligrams per liter.

These nitrate concentrations are relatively high due to the fact that Limestone Run flows through large amounts of agricultural land.

[1] The concentration of chlorides in the waters of Limestone Run and its tributaries ranged from 8.2 to 21.5 milligrams per liter on September 20, 2003.

[1] The concentration of manganese in Limestone Run ranges from 24.9 to 71.2 milligrams per liter at the stream's mouth and source, respectively.

The concentration of cadmium in Limestone Run is so low as to be undetectable in some locations, but is as high as 0.06 milligrams per liter in Lewisburg.

[4] Much of the southern part of the Limestone Run watershed is in the Wills Creek formation, which comes from the Silurian period and consists mainly of calcareous shale.

The part of the watershed is composed of rocks from the Bloomsburg and Mifflintown formations, which mainly consist of shale.

The Keyser and Tonoloway formations, which mainly consist of limestone and are from the Devonian and Silurian periods, are found in the northern, central, and extreme southern parts of the watershed.

In 2012, workers overseen by the Union County Conservation District did work to stabilize the banks of Limestone Run and altered the channel of the stream.