Lochloosa is an unincorporated community in Alachua County, Florida, United States.

The community is located on the eastern shore of Lochloosa Lake along U.S. Route 301, 5.6 miles (9.0 km) south of Hawthorne.

Lochloosa has a post office with ZIP code 32662, which opened on September 9, 1881.

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