The hospital is off of the Masaka-Mbarara Road, in the town of Lyantonde in Lyantonde District, approximately 67.5 kilometres (42 mi), by road, east of Mbarara Regional Referral Hospital.

[1] This is about 73 kilometres (45 mi) west of Masaka Regional Referral Hospital.

[2] The coordinates of Lyantonde General Hospital are 0°23'56.0"S, 31°09'13.0"E (Latitude:-0.398889; Longitude:31.153611).

[3] Before 2011, Lyantonde General Hospital was a Health Centre IV.

In 2011, the health center was elevated to a full hospital.