Madonna in Glory with Seraphim is a tempera painting on panel by the Italian Renaissance painter Sandro Botticelli, executed c. 1469–1470.

The art historian Wilhelm von Bode first attributed it to Botticelli and dated it to the Verrocchio phase of his early career, c. 1469–1470 and just before his Fortitude.

Later studies confirmed von Bode's hypothesis, with the exception of Adolfo Venturi who believed it to come from the school of Filippo Lippi.

Botticelli has succeeded in expressing the tensions in this theme with sensitivity: the mother, who is fully aware of the Passion her son will suffer, is holding him protectively in her arms.

Similarly, Marie's delicately rounded face depicted with contour lines also recalls Lippi's work.