It encompasses parts of Kentucky Routes 70 and 255 within the park in northwestern Barren and eastern Edmonson Counties.
It closely follows the Mammoth Cave Railroad Bike & Hike Trail.
The road continues northward to the junction with Kentucky Route 70 (KY 70) near the Barren-Edmonson County line within the Mammoth Cave National Park.
A little beyond the Sloan's Crossing Pond Trail, KY 70 westbound leaves the parkway by making a left turn towards Brownsville, while the Mammoth Cave Parkway continues north to end at the Visitor's Center beyond the crossroad intersection with East Entrance and Carmichael Roads and intersections with Green River Ferry and Flint Ridge Roads.
[3] The segment between Brownsville Road and Green River Ferry Road is part of the Duncan Hines Scenic Byway, one of the routes of the Kentucky Scenic Byways system.