Provincial Road 259 (PR 259) is a secondary highway in the Canadian province of Manitoba.

The route, located in southwestern Manitoba is 64.2 kilometres (39.9 mi) long.

PR 259 begins near Virden, at a crossroads junction with the Trans-Canada Highway (Provincial Trunk Highway 1, PTH 1), where it continues southward as PTH 83.

At its junction with PR 254, PR 259 turns northward, and then curves east to PTH 21 at Kenton.

It reaches its eastern terminus at PR 250 near Wheatland shortly after its PTH 25 junction.