Numerous characters have been added in the game such as; Koopa Troopa in July 2018, Blooper in August 2018, Diddy Kong in September 2018, Birdo in October 2018, Koopa Paratroopa in November 2018, Petey Piranha and Shy Guy in December 2018, Luma in January 2019, Boom Boom in February 2019, Pauline in March 2019, Kamek in April 2019, Dry Bones in May 2019, Fire Piranha Plant in June 2019, and Dry Bowser in July 2019.

[9] However, players are able to counter a zone shot without taking damage using a "block", which can be performed by hitting the ball with perfect timing.

[13] Players can unlock additional outfits and characters by participating in special online Tournaments held by Nintendo.

[15] Wario and Waluigi are mining at the Temple of Bask when they discover a treasure chest containing Lucien, a magical racket said to be legendary to those who would possess it.

The duo perceive themselves as displaying pride at matches using Lucien, and open up the treasure chest to unveil it.

The duo attempt to offer Lucien to Mario, but Luigi snatches it up, after which it begins to take possession of him, Wario, and Waluigi, through use of its grip tape.

Aster tells the backstory of Lucien and how many years ago, King Bask stripped Lucien's power and divided it among five Power Stones hidden on the island, and years later the room fell to ruin, breaking the seal and allowing the racket to fall into new hands.

Wario and Waluigi decide to settle the score at Marina Stadium; whoever wins receives all five Power Stones.

They win, allowing them to receive the Power Stones, but Bowser gets ahold of Lucien before this can happen, and makes off to the Temple of Bask with it.

Although praise went to its new mechanics for making the game "far more fun, well balanced and less gimmicky brand of superpowered tennis than that of its disappointing Wii U predecessor, Mario Tennis: Ultra Smash," Aces' adventure mode was criticized for feeling underdeveloped and not having enough replay value (such as harder versions of challenges after completing it) or unlocks, and that the main multiplayer modes lacked court selection and local tournament functions.

[27] By March 2019, total sales had reached over 2.64 million copies, making it the best-selling Mario Tennis game.

[29] The 2022 CESA Games White Papers revealed that Mario Tennis Aces had sold 4.28 million units, as of December 31, 2021.