MD 341 begins at an intersection with MD 16 (Church Creek Road) and Maple Dam Road near the southern edge of the city of Cambridge adjacent to Cambridge-South Dorchester High School.

Maple Dam Road heads south toward Blackwater National Wildlife Refuge.

MD 16 heads west toward Church Creek and east on a bypass of Cambridge toward U.S. Route 50.

The state highway reaches its northern terminus at an intersection with MD 343 (Washington Street).

[1][2] Race Street south of Washington Street was included as part of the Cambridge–Church Creek highway designated for improvement as a state road by the Maryland State Roads Commission in 1909.