He played college football for the Florida Gators, where he was a member of a BCS National Championship team, recognized as a consensus All-American, and won the 2009 Rimington Trophy, awarded annually to the best college football center.

[8] Beginning in 2008, Pouncey became the full-time starting center for Florida next to his twin brother Mike at right guard.

[8] In the season opener against Charleston Southern, he began 2009 with a grade of 98% and had two knockdowns in the game.

During the week 4 match-up against Kentucky, he would end with a grade of 90% and receive Champion Club Honors.

[9] He was also voted a first-team All-American by CBSsports.com, Sporting News, Football Weekly, and Walter Camp.

[16] In his rookie year, he started in all 16 games played at the center position, and was selected to the Pro Bowl.

[19] In the AFC Championship, Pouncey injured his ankle during the win over the New York Jets and was not able to play in the Super Bowl.

[16] This extension made him, at that time, the highest paid center in the National Football League.

[29] After a successful recovery from knee surgery, Pouncey started all 16 games in 2014 and was selected to the Pro Bowl for the fourth time of his career.

[32] On August 23, 2015, during the Steelers' first preseason game, Pouncey suffered a broken fibula when Green Bay Packers safety Ha Ha Clinton-Dix rolled onto the back of Pouncey's ankle.

[35] Pouncey was named to his fifth career Pro Bowl in recognition of his 2016 season.

[36] On December 19, 2017, Pouncey was named to his sixth Pro Bowl along with fellow Steeler offensive linemen Alejandro Villanueva and David DeCastro.

[37] On March 7, 2019, Pouncey signed a three-year, $33 million contract extension with the Steelers through the 2021 season, making him the highest-paid center in the NFL.

[38] On November 14, 2019, during Thursday Night Football against the Cleveland Browns in Week 11, Pouncey was involved in a brawl with Myles Garrett.

Pouncey threw punches and kicked Garrett, resulting in the two getting ejected.

[43] On February 12, 2021, Pouncey announced his retirement, alongside his brother, after an 11-season career.