Methanol dehydrogenase

[2] This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

[2] For Gram-negative bacteria, methanol oxidation occurs in the periplasmic space, facilitated by PQQ-dependent MDH.

PQQ-dependent MDHs contain a PQQ prosthetic group, which has the role of capturing electrons from methanol oxidation and passing them to the cytochrome.

[4] Oxygen-dependent alcohol oxidase (AOX) can be obtained from eukaryotic methylotrophs in the peroxisome of yeasts.

Dihydroxyacetone synthase (DAS) and catalase (CTA) must then transform these toxic chemicals into non-toxic forms to protect the cell.

PQQ-MDH enzyme structure, with PQQ in the center. The yellow sphere represents a La 3+ ion. [ 1 ]
Shows the reaction of methanol to formaldehyde catalyzed by MDH. [ 2 ]