In enzymology, a methionyl-tRNA formyltransferase (EC 2.1.2.9) is an enzyme that catalyzes the chemical reaction This enzyme belongs to the family of transferases that transfer one-carbon groups, specifically the hydroxymethyl-, formyl- and related transferases.

The systematic name of this enzyme class is 10-formyltetrahydrofolate:L-methionyl-tRNA N-formyltransferase.

This enzyme participates in three metabolic pathways: methionine metabolism, one carbon pool by folate, and aminoacyl-tRNA biosynthesis.

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1FMT and 2FMT.

This EC 2.1 enzyme-related article is a stub.