The second segment runs from French Camp at MS 413 west (but again signed north-south) to Winona at U.S. Route 51 (US 51).

Upon entering Weir, the road makes a slight curve to the northeast and becomes known as South Ann Street as it passes through a residential neighborhood.

MS 407 heads west northwest (but signed as north) out of the town limits past a few homes and between a pair of high voltage electrical substations.

The highway travles through a rural area, intersecting the unsigned MS 43 at its northern terminus in the county.

The road briefly enters the northeasternmost portion of Carroll County where it heads through an unnamed settlement centered about its intersection with CR 430.

The state highway enters the city of Winona, but in its far reaches where sparse housing and some open fields are found along the road.

Before ending, MS 407 gains a center turn lane and passes an elementary school before reaching a T-intersection with US 51.