Monsampolo del Tronto is a comune (municipality) in the Province of Ascoli Piceno in the Italian region Marche, located about 80 kilometres (50 mi) south of Ancona and about 18 kilometres (11 mi) southwest of Ascoli Piceno.

Monsampolo del Tronto borders the following municipalities: Acquaviva Picena, Castorano, Controguerra, Monteprandone, Offida, Spinetoli.

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